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PoultryStar® is a well-defined, multi-strain Direct Fed Microbial (DFM) for poultry, and contains live, viable, naturally occurring microorganism (probiotics) which are categorized and classified as Generally Recognized As Safe (GRAS). PoultryStar® was developed in the course of a multinational research project partially funded by the European Union. In the course of the project, numerous intestinal bacteria were isolated from the gut of several healthy chickens and thoroughly characterized by combining morphological, physiological and genotypic methods. The most promising strains were evaluated for important probiotic criteria such as adhesion capability to intestinal cell lines, inhibition of pathogens (e.g. S. enteritidis, S. typhimurium, S. choleraesuis, C. jejuni, E. coli and Cl. perfringens), immunological activity, range of metabolic end products like Volatile Fatty Acids (VFA), fermentation performance, stability against acids and bile salts, storage stability and safety status. Based on these results a synbiotic product consisting of strains belonging to the genera Enterococcus, Pediococcus, Lactobacillus, Bifidobacterium and prebiotic fructooligosaccharides has been developed.